World Politics an Example by

World Politics THERE is an old saying that goes something like this: It makes a difference whose ox is being gored. The point of this saying is that the same event can look different to different people. It depends upon each ones background and interest in the matter. It is usually easier for a person to be generous and unprejudiced about a problem as long as he is not too closely involved. But what happens if the problem moves into his own backyard, as it were? Then he may be prone to view it in a different light. Need essay sample on "World Politics" topic? We will write a custom essay sample specifically for you Proceed Nations are like people in this respect, except that their attitudes affect millions of persons and the course of world history. As long as a problem does not touch a nation directly, it can be very noble and generous in its outlook. But as soon as the national interest is directly involved, it may quickly change its stance. So it is that two countries can take completely opposite views of the same problem or point of history. However, this does not mean that one (or both) is deliberately lying, though that could be the case. Indeed, they may not even be aware that they are presenting almost totally different interpretations of the same events. A look at a few historical situations will reveal that this is true. It will help readers to appreciate that misunderstandings between nations are almost unavoidable under todays political systems. It also emphasizes how badly all mankind needs a better system of government. The average citizen of the United States is aware of certain facts about Canada, the vast country that lies to his north. But his information may not go much beyond knowing that there are descendants of the British, French and Americans living there, that they see the northern lights, and that the Mounted Police always get their man. If he is old enough he may have heard about the Dionne quintuplets. One of such facts the average American assumes is that for the past two centuries Canadians as a whole have loved Americans. Indeed, he may feel that Canadians would no doubt have been willing to become a part of the United States if the British or Canadian governments would have permitted it. Beyond this knowledge, there are many other things that the US government doesnt know about Canada. However, with the existing relationship within Canada and the US today, it is pretty obvious how the two countries have tried a lot in mending the shortcomings of both governments to each other. Through the International trade, there has been competition between the two, but amidst the economic struggling that happens in the International trade; both countries try to connect the differences they have against each other. Politically, there are still some existing misunderstandings between Canada and the United States. Although this is true, many analysts believe that both governments would be able to make amends with all these issues because of diplomacy and the application of human rights law and other international relationship principles. Indeed it is still undeniable that Political conflicts usually occur between neighboring countries especially with regards to economic struggles. However, if diplomacy is considered, these conflicts could be given way and set aside for some time, for the sake of the bigger public. Question: What are some of the most serious threats to democracy in Latin America today? TODAY, many Third World countries in Africa, Asia, and Latin America are impoverished and hindered in countless ways. People in many of these lands accuse their existing governments and local religious leaders of oppression. Others blame their current problems on foreign debt. Today, aside from all the oppression and governments authority abuse as claimed by some Latinos, it is quite sure that the government of Latin America and the society living around it is alarmed in living less democratic lives in the near future. Among the reasons behind this is the Political situation within the territories of the said country. According to Donald B.Schulz in his article The growing threat to democracy in Latin America, he said that Perus troubled electoral processes in the year 2001 indicates the on going threat to the Latin American democracy. Among the main reasons referred to by political analysts is the justice development of Latin America. With the development of the judicial processes and contents of the laws of the Latin American governments, it is quite obvious how the enhanced status of the Latin American laws have directly affected the democracy of the society of the said social section. Certainly, anything that is too much is not good. Even laws which are supposed to secure the human community begin to have less effective ways of protecting the society once it is abused. Indeed, everything should remain in a balanced way especially with regards to social acceptance. Bibliography Stanley Hoffman. (2002). Clash of Globalizations. Council of Foreign Relations Incorporated. Schulz, Donald. (2001). The Growing Threat to Democracy in Latin America. https://www.questia.com/read/1G1-73000542/the-growing-threat-to-democracy-in-latin-america. (October 7, 2006).

Buddhism and Islam throughout the world Essay

Buddhism and Islam throughout the world Essay Buddhism and Islam throughout the world Essay Michael Ahn Fung Laoshi The Buddha: His life and Teachings 2/25/13 Short-term Paper Mahayana, meaning â€Å"Great Vehicle†, is considered itself as a more authentic version of Buddha’s teachings. Mahayana Buddhism is essentially a vision of what Buddhism is really about and contains a vast corpus of philosophical and devotional texts and Buddha’s teachings. One of the most distinctive themes in the Mahayana Buddhism is Emptiness of mind. According to the Mahayana teaching of Emptiness, sunyata, refers void or openness, symbolizing that all beings and phenomena have no intrinsic existence in themselves. From Mahayana teachings, Emptiness is the understanding that the things of the phenomenal world do not exist as separate, independent and permanent entities, but rather appear as the result of an infinite number of causes and conditions, are a product of dependent origination. From the Mahayana Buddhism, Sunyata refers to the fact that everything is dependently originated, including the causes and conditions. â€Å"All phenomena are dependent for their existence on complex networks of causes and conditions† (pg.26). For example, suppose there is a book. A Book is dependent on the printing press that printed it, and dependent for its existence on the papers and ink that constitute the book. Printing press, ink and papers are dependent to each other to create the book itself. There is no inherent book essence inhabiting the ink and papers. The material is not the book, the shape is not the book, and the function is not the book. All these combined features make up the book. In addition, Emptiness means no permanent and unchangeable existence. â€Å"All phenomena are empty of essence, but exist impermanent† (pg.26). For instance, the human body is changing every second like any phenomenon. It does not have permanent and unchangeable existence. Body can be the emptiness. From the Buddhism teachings, this is the truth of nature which is a continuous process. Beings and phenomena arise and perpetuall y cease for other new beings and phenomena. Rising and falling are the common characteristics of worldly existence. All phenomena are always in the cycle of rising and falling. Thus the truth of sunyata is the nature of each individual existence. Each step in understanding that each minor form has a nature that is not described by words is steps to the realization of sunyata. According to Buddhist teachings, when people realize that we ourselves are empty, freedom from suffering accomplished. The fourth noble path is closely related to sunyata. In fourth noble path, the third one is â€Å"the cessation of suffering is attainable.† From this path, it states that the state of Nirvana means freedom from all worries, troubles, complexes, fabrications and ideas. The state of Nirvana can be reached when the emptiness is

5 Rules for Using Logic to Order Lists

5 Rules for Using Logic to Order Lists 5 Rules for Using Logic to Order Lists 5 Rules for Using Logic to Order Lists By Mark Nichol At least five factors dictate how items in an in-line list a series of items within a paragraph, as opposed to a vertical list are organized. (See an earlier post about in-line lists.) It’s all about the context: alphabetization, chronology, complexity, interrelationship, or sequence. (Guess which context I chose for the preceding sentence.) 1. â€Å"Our shop specializes in teak, ebony, and mahogany furniture.† The store may stock more teak than ebony and more ebony than mahogany, or the order may reflect relative prices, but the sentence does not explicitly or implicitly express either idea. In such ambiguous cases, alphabetical order is an appropriate default setting: â€Å"Our shop specializes in ebony, mahogany, and teak furniture.† 2. â€Å"The major US wars of the nineteenth century were the Mexican-American War, the Civil War, and the War of 1812.† The presentation of information in this sentence does not justify the order in which the list items are given. If it specifically referred to the relative cost in human lives or in dollars, for example, then the sequence would follow that theme, but in the absence of an obvious context, reference to historical events should be chronological: â€Å"The major US wars of the nineteenth century were the War of 1812, the Mexican-American War, and the Civil War.† 3. â€Å"Among his favorite musical pastimes are drumming in a world music ensemble, yodeling, and playing the harpsichord.† Again, absent a contextual framework for a list, it’s best to impose order. In this case, the somewhat amorphous descriptions don’t readily admit an alphabetical scheme, so perhaps, for euphony, the items should be arranged from simplest to most complex in terms of syllabication: â€Å"Among his favorite musical pastimes are yodeling, playing the harpsichord, and drumming in a world music ensemble.† 4. â€Å"It is allowed in some countries, forbidden in a few, and tolerated in others.† This list describes three degrees of tolerance for a certain policy, so the items should be listed in an ascending or descending order: â€Å"It is allowed in some countries, tolerated in others, and forbidden in a few.† 5. â€Å"Take a look at the map, and you will see that Scandinavia consists of Norway, Finland, Denmark, Iceland, and Sweden.† Without the reference to the map, this sentence could be organized by any one of several schemes, including alphabetical order, geographical location, and relative size. But because speakers of English generally read left to right, and maps are usually oriented to the north, a west-to-east organizational scheme seems most appropriate: â€Å"Take a look at the map, and you will see that Scandinavia consists of Iceland, Denmark, Norway, Sweden, and Finland.† (OK, Denmark’s westernmost point is slightly to the east of Norway’s, but would you separate the twins Norway and Sweden?) These contexts are not always mutually exclusive: In a sentence like â€Å"The Olympic medals are gold, silver, and bronze,† the scheme could be described as one of interrelationship (gold is more valuable than silver, which is more valuable than bronze) or sequence (gold is for first place, silver for second place, and bronze for third place). But that’s not the point; the idea is to provide some framework any framework for a list so as not to distract the reader. Sometimes, a list’s lineup is determined more by tradition than anything else: â€Å"The original Three Stooges consisted of Moe, Larry, and Curly.† Moe was the leader of the group, but there’s no reason to mention Larry before Curly rather than the other way around except that it seems to trip off the tongue more easily that way. Sometimes, that’s as good a reason as any. Want to improve your English in five minutes a day? Get a subscription and start receiving our writing tips and exercises daily! Keep learning! Browse the Style category, check our popular posts, or choose a related post below:Coordinating vs. Subordinating Conjunctions50 Synonyms for "Assistant"Ebook, eBook, ebook or e-book?

Fracking Essay Example | Topics and Well Written Essays - 2000 words - 1

Fracking - Essay Example Proponents of fracking, on the other hand, believe that fracking promotes the economy and that the regulations on trafficking are enough to meet the concerns over fracking. This research essay will analyze the positive and negative political, social, economic and environmental effects of fracking. US has unlimited exports of gas especially in Ohio, Colorado and Pennsylvania, which have a number of implications to the economy. The gas producing states have experienced benefits associated with fracking that include lease payments, cheap energy, jobs and royalty checks. The gas industry has showed a revolutionary potential through creation of energy markets globally. There is high rise in demand for the natural gas prices in the international markets following the rising demands on the use of natural gas as an alternative form of energy. Fracking has brought more benefits to the concerned states. In Ohio, the state’s economy has benefited from fracking for many areas in the form of include taxation among other associated benefits. Additionally, fracking has increased the availability of jobs; research shows that Ohio counties jobs are found in fracking major industries and such jobs include pipeline constructions and drilling, which contribute to 15.5% total employment op portunities in the country. Pennsylvania and Colorado‘s economies have benefitted from fracking. Part of their economy is attributed to gas drilling, which earns the states a lot of revenues necessary for the development of other economic sectors. Unemployment sector has also benefited from the creation of jobs opportunities. Additionally, shale gas opportunities have increased and today, the state of Ohio, Pennsylvania and Colorado has registered the millions of money resulting from the gas drilled from the lands that the citizens lease. Many Americans have leased their lands to the gas extracting industries which in turn earns them more money

Juno the Movie Review Example | Topics and Well Written Essays - 2750 words

Juno the - Movie Review Example She is headstrong when she wants to be and this is apparent in the decisions she takes as a teenager saddled with an unplanned pregnancy. The father is her classmate Paulie Bleeker who takes life as it comes. The story unfolds with Juno deciding to give up her child to an urban couple - Mark and Vanessa Loring. This couple goes through a metamorphosis as they try to convince Juno regarding the fact that they would be perfect parents to her child. On the other hand, Juno has to break the new to her parents - stepmother Bren, and father Mac. Juno's parents are a classic example of a couple who strive to keep a close knit family and try to set the right example for their children. What follows here is a confrontation between Juno's parents and the prospective foster parents of her unborn child. This is where the drama element of the movie takes off in order to cater to the following elements: In this paper, these issues will be discussed in terms of an evidence based theory that is based on the foster care system in cases of divorce, teen pregnancy and other such developmental issues within a child. The foster care system in the United States continues to face challenges related to the quality of care provided by foster parents. Although legislation has been passed by the federal government aimed at improving the system, the pervasive negative stereotypes of foster care remain. This is especially true when it comes to the development and family environment of children placed in this environment. In an effort to better understand the development and family environment issues of children in foster care, this research seeks to develop a more integral comprehension of how children in foster care view their own personal development and family environment. With this information, it will be possible to better understand specific development and family environment issues indicative to foster care. In addition, this data will elucidate development and family environment issues that may not be persistent concerns for children in foster care. With this data, social workers could develop p rograms to raise awareness and improve development and family environment outcomes for children in this care setting. To begin with, a foster home has been described as that place where a child is handed over to responsible adults, duly analyzed and hand picked by various relevant authorities. The perception and conception of the various facets of a child's life are built here accordingly depending on the various elements like education, relationships and others. These go on to form a child's perception of aspects like development and family environment and security. Besides these parameters, the paper follows the guidelines that are embedded in the basic conceptual framework followed by the researcher. These have to do with the guidelines and concepts laid down by authorities like WHO. The US Department of Health and Human Services, Administration for Children and Families (2005) reports that at the end of 2004, 800,000 children were served by the foster care system. Of these children, 304,000 represented new entrants into the program. Although this number is part of a larger trend representing a

Tag-derived Simple Sequence Repeat Markers of Olive

Tag-derived Simple Sequence Repeat Markers of Olive Identification and functional annotation of expressed sequence Tag-Derived Simple Sequence Repeat Markers of olive (Olea europaea) Olive tree (Olea europaea L.) is one of the most important oil producing crops in world, the genetic identification of several genotypes by using molecular markers is the first step in breeding programs. A large number of Olea europaea expressed sequence tags (ESTs) 11,215 were done from the NCBI database and used to search for microsatellites. Our result Explained that 8295 SSRs were present and its percentage of occurrence which about 77.6%,11.84%,8.62%,0.84%,0.77% and 0.29% for Mononucleotide, trinucleotide, dinucleotide, hexanucleotide, pentanucleotide and tetranucleotide respectively. The appearance of the AAG/CTT repeat was highly percentage in trinucleotide and percentage of AG/CT was highly in dinucleotide repeats. By using flanking region of SSRs repeat we designed 1,801 EST-SSR primer pairs. The result obtained from Functional annotation of olive EST sequences containing SSRs indicated that 81% of these sequence having homology with known proteins, while 1.55% was homologou s to hypothetical or unknown proteins and the 17.37% sequences did not possess homology with any known proteins. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation revealed that EST containing SSRs were implicated in diverse biological process include cellular and metabolic process, while in molecular function includes catalytic activity, binding and enzyme regulator activity. A total of 93 different pathways were significant matches in the KEGG database, which divided onto Carbohydrate metabolism such as glycolysis/gluconeogenesis pathway and the Energy metabolism such as Carbon fixation in photosynthetic organism pathway, also this included 11 different pathways from Lipid metabolism such as Fatty acid biosynthesis pathway. We isolate a genomic DNA from 9 olive cultivars and tested with 25 random selected primer pairs for amplification and polymorphism detection. All tested primers, exhibited successfully amplified and detected polymorphism. Olive tree (Olea europaea L.) is one of the most superannuated and important long lived fruit species in Mediterranean [1], its diploid species (2n = 2x = 46), and the genome size range between 2.90 pg/2C and 3.07 pg/2C, with 1C = 1,400 1,500 Mbp [2]. Olea europaea is one of the first domesticated crops from Oleaceae family, and it cultivated for table olives and edible oil [3], a long history of olive cultivation in the Middle East was descriptions by archaeology and botanists [4]. The olive cultivars are high of number that more than 1200 cultivars [5], also the accessions are available in a large numbers in olive producing countries, that occurrence a problems for germplasm preservation and it management [6]. The genetic identification and characterizing of several genotypes by using molecular markers is the first step in breeding programs [7], and by increased rate of mutation in microsatellites repeats that show a highly level of length polymorphism [8]. With the improvement and increasing of DNA sequencing technology, sequencing of expressed gene are used to obtain a large collection of EST which are isolated from a specific tissue and stage on organism [3]. Recent EST-SSR studies have reported that the EST is uses a source of SSRs and that reveal highly polymorphism [9]. EST sequences Available in public database and by using bioinformatics tools can determine and development of SSR markers in that EST sequences [10]. In olive that can be allow to development of new functional marker and use it in molecular breeding [11]. Also it can use as useful tools for gene and marker discovery, gene mapping and functional comparative studies. EST-SSRs recently reported in several plant species, such as Musa [12], Finger Millet [13], Jatropha Curcas [14], Pineapple [15], Celery [16], Lettuce [17], Barley [18], Radish [19], Citrus [20], Watermelon [21], Sugarcane [22], grapes [23], Cereal species [24] and bread wheat [25]. A large number of EST sequences in olive are available on database it can be a useful resource to develop gene based markers. The aim of this study was to use bioinformatics tools to develop and identify a new genic marker EST-SSR in Olive, to compare the frequency and distribution of different repeat types in genic sequences. Also determine the localization of these primers in different pathways in plant, to use it as tools to differences between the olive cultivars. The Source of Sequence, screening and primer designing of microsatellites. EST database used a source of olive EST sequences from NCBI (http://www.ncbi.nlm.nih.gov). A total of 11,215 ESTs sequences of Olea europaea are available and used in this study. Identification of SSRs by using the perl script MISA (MIcroSAtellite identification tool; http://pgrc.ipk-gatersleben.de/misa/).The criteria used to determine SSR repeat were: mononucleotide ≠¥ 10, dinucleotide ≠¥ 6, trinucleotide, tetranucleotide, pentanucleotide, and hexanucleotide ≠¥ 5, and the maximal number of bases interrupting two SSRs in a compound microsatellite are 100 bp. The flanking region of SSR motifs used to design SSR primers by using primer3_core [26]. The parameters used were: optimum length of primer 20 nucleotides, optimum annealing temperature (Tm) of 58 °C, expected amplified products size of 100-500 bp and optimum G/C content 50 %. Validation of designed primer For primer validation, we designed 25 EST-SSR primers and test these primers on 9 olive cultivars. Total genomic DNA was extracted from olive leaves using Plant Genomic DNA Kit (QiGen). PCR amplification was conducted in 25  µ l reactions containing 50 ng of template DNA, 2.5 mM MgCl2, 5  µ l 5X PCR buffer, 0.5 mM each primer, 0.5 U Taq DNA polymerase, and 2.5 mM dNTPs. The PCR cycling profile was 94 °C for 5 min, 35 cycles at 94 °C for 45 s, the optimum annealing temperature for each primer pair shown on (Table S1) for 50 s, 72 °C for 45 s, and a final extension at 72 °C for 10 min. The quality of the PCR product was checked by mixing it with an equal volume of loading buffer and then visualizing the band on a 1.5% agarose gel in TBE buffer at 100 W for 120 min. Identification of EST-SSRs Putative Function annotation The putative function annotation of EST sequences contains SSR performed by used Blast2go program [27] to BLAST against a reference database. Also blast2go program are containing many features such as Gene Ontology (GO), Enzyme Commission (EC), and KEGG annotation. Distribution of various repeat type in olive Our result referred to 4,088 EST sequences about 36.45% from 11,215 of Olea europaea EST sequences as containing 8,295 various motif SSRs that Due to the EST sequences maybe contain more than one SSR motif (Table 1), and this number based on the criteria we used it to identify SSR motifs in the EST sequence. The investigation of different types of SSR repeats in our result showed that the highest percentage of appearance mononucleotide repeats were 77.64%, followed by trinucleotide 11.84%, dinucleotide 8.62%, hexanucleotide 0.84%, pentanucleotide 0.77% and tetranucleotide 0.29% (Fig. 1). The higher abundant of trinucleotide in coding regions were consistent with the previous studies in eukaryotic genomes [28, 31]. In mononucleotide A/T repeats 88.8% were higher than the G/C 11.2% motifs, and these results were proportionate with SSRs analysis of chloroplast SSRs on Olea species [29] and with SSRs analysis of major cereal organelle genome [28]. GA motifs were representing 55% from dinucleotide motifs in olive EST sequences. According to previously studies from foxtail millet [31], barley, maize, rice, sorghum and wheat [30], GA motifs were the most abundant motifs in these crops. AG/CT and GA/TC motifs were the most frequent respectively and CG motifs the lowest frequencies were found in olive, this case reported in the distribution of microsatellites on three different plant families that Brassicaceae, Solanaceae and Poaceae [32]. Dinucleotide motif can represent to multiple codons that depending on the reading frame and can translate into different amino acids such as, AG/CT motif can represent AGA, GAG, CUC and UCU codons in mRNA and translate into the amino acids Glu, Arg, Leu and Ala respe ctively, Ala and Leu were present in protein at higher frequencies, hence the higher incidence of GA, CT motifs in the EST sequences [33]. This could be one of the reasons why GA, CT motifs are present at such highly appearance in EST collections [34], dinucleotide repeats that located on coding regions are more sensitive to any change such as any addition or deletion because that causes a frame shifts and will give different amino acids [35]. As for trinucleotide TCT, TTC were the most common repeat motif in olive EST (Table 2), While AAG/CTT motifs were the most common in chloroplast of Olea species SSRs [29], however, in other crops such as barley, maize, rice, sorghum and wheat CCG or AAC were the most common trinucleotide repeat [30]. Our results revealed that tetranucleotide motifs AATC, CTTT are the most common; however the most common in Olea species SSRs chloroplast were AAAG, CTTT [29]. Pentanucleotide and hexanucleotide AAAAT and GAAAAA respectively are the most common repeat motif in our results while [29] found AATCC was the most common on pentanucleotide in Olea species SSRs chloroplast and hexanucleotide was not found. Design and validate of EST-SSR In this study, we designed 1,801 PCR primer pairs from the 8295 SSR motifs of Olea europaea EST, The designed primers were referred as Oe-ESSR_xxxx, where Oe-ESSR referred to Olea europaea EST SSR, xxxx was referred the number of EST-SSR from 1-1801 and that different of the previously SSR primers designed from chloroplast sequences of Olea species [29]. This primers were listed in the (Table S1), and provide with all information related it such as Primer name, GenInfo Identifier gi number of EST sequence, Repeat type, Repeat Sequence, Length of Repeat, Repeat start on sequence, Repeat end on sequence, Forward and Reveres Primer, Tm ( °C), Length of Primer (bp), product Length (bp), sequence of EST, Sequence Description, gene ontology, Enzyme code and Enzyme Name. We use a sample of 25 primers randomly from these 1,801 EST-SSR primers to validate it by using a genomic DNA isolated from 9 olive cultivars. All tested primers, exhibited successfully amplified and detected polymorphism (Fig. 2). Putative Function annotation of EST-SSRs The putative function annotation of the EST sequences containing SSR performed by used Blast2go program [27]. According to the Blast2go result, 81% from EST sequence as homology with known proteins, while hypothetical or unknown proteins were 1.55%, and 17.37% of this EST sequences did not homology with any known proteins. The gene ontology of olive EST sequences containing SSRs using Blast2GO revealed that in the biological processes, the highly appearance of SSR were involved in cellular processes, metabolic, response to stimulus, biological regulation and developmental process, while Signaling, rhythmic processes and growth had the lowest SSR contents among these EST. The molecular function category includes catalytic activity and binding, while cell membrane and organelle were assigned in the cellular component category (Fig. 3). The Similar results were found on functional annotation of the citrus and date palm EST sequences containing SSRs [20, 36]. Our results agreement with t he similar results obtained in [20] which suggested that genes were involved in protein metabolism and biosynthesis were well conserved in plants. Functional classification by KEGG pathway analyses The KEGG Pathway analysis is useful tool to understand the molecular interaction and biological functions [37]. Our study exhibited a total of 93 different pathways include 253 enzymes target by 381 EST-SSR primers were significant matches in the KEGG database (Table S2), this data can Visualization by using circos software [38] (fig. 4). The higher occurrence of SSR on pathways indicated a good potential for using these molecular markers to targeting the enzyme related to the trait subjected in our study. This EST sequences contain SSR were categorized into metabolism, as well as its subcategories, including lipid metabolism (Table 3), carbohydrate metabolism, energy metabolism, amino acid metabolism, nucleotide metabolism and metabolism of cofactors and vitamins. In details, the mapping result can further investigated against the glycolysis/gluconeogenesis (Fig. 5), Oxidative phosphorylation (Fig. 6) and Fatty acid degradation (Fig. 7) pathways as an example of Carbohydrate metabolism, Energy metabolism and Lipid metabolism respectively. Conclusion SSR markers are very important because it is co-dominant, highly polymorphic and can generate from functional regions of the genome. EST-SSR technique have the potential to generate phenotypically linked functional markers and a useful tool can use on genetic diversity, marker assisted selection and genome mapping in olive. This study exhibited the functional categorization of olive EST sequences containing SSR revealed that these ESTs representing in genes with cellular component, biological process and molecular function. This EST-SSR primers also providing with useful information to understand the biological functions and genes interactions according to the localization of this primers in different pathways related to possible phenotypic differences between the olive cultivars. References Gaby E, Mbanjo N, Tchoumbougnang F, Mouelle AS, Oben JE, Nyine M, et al. Development of expressed sequence tags-simple sequence repeats ( EST-SSRs ) for Musa and their applicability in authentication of a Musa breeding population. Afr J Biotechnol. 2012;11(71):13546–59. Naga BLRI, Mangamoori LN, Subramanyam S. Identification and characterization of EST-SSRs in finger millet (Eleusine coracana (L.) Gaertn.). J Crop Sci Biotechnol. 2012;15(10):9–16. Wen M, Wang H, Xia Z, Zou M, Lu C, Wang W. Development of EST-SSR and genomic-SSR markers to assess genetic diversity in Jatropha Curcas L. BMC Res Notes. 2010;3:42. Wo T. In silico mining for simple sequence repeat loci in a pineapple expressed sequence tag database and cross-species amplification of EST-SSR markers across Bromeliaceae. Theor Appl Genet. 2011;123:635–47. Fu N, Wang PY, Liu XD, Shen HL. Use of EST-SSR markers for evaluating genetic diversity and fingerprinting celery (apium graveolens l cultivars. Molecules. 2014;19:1939–55. Simko I. Development of EST-SSR markers for the study of population structure in lettuce (Lactuca sativa L.). J Hered. 2009;100(2):256–62. Zhang M, Mao W, Zhang G, Wu F. Development and characterization of polymorphic ESTSSR and genomic SSR markers for tibetan annual wild barley. PLoS One. 2014;9(4):1–10. Nakatsuji R, Hashida T, Matsumoto N, Tsuro M, Kubo N. Development of genomic and EST-SSR markers in radish ( Raphanus sativus L .). Breed Sci. 2011;61:413–9. Liu S, Li W, Long D, Hu C, Zhang J. Development and Characterization of Genomic and Expressed SSRs in Citrus by Genome-Wide Analysis. PLoS One. 2013;8(10):1–10. Campus P. Development of EST-SSRs in watermelon (Citrullus lanatus var. lanatus) and their transferability to Cucumis spp. J Hortic Sci Biotechnol. 2008;83(6):732–6. Pinto LR, Oliveira KM, Ulian EC, Garcia AAF, de Souza AP. Survey in the sugarcane expressed sequence tag database (SUCEST) for simple sequence repeats. Genome. 2004;47:795–804. Scott KD, Eggler P, Seaton G, Rossetto M, Ablett EM, Lee LS, et al. Analysis of SSRs derived from grape ESTs. TAG Theor Appl Genet. 2000;100:723–6. Varshney RK, Thiel T, Stein N, Langridge P, Graner A. In silico analysis on frequency and distribution of microsatellites in ESTs of some cereal species. Cell Mol Biol Lett. 2002;7:537–46. Gupta PK, Rustgi S, Sharma S, Singh R, Kumar N, Balyan HS. Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat. Mol Genet Genomics. 2003;270:315–23. Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, et al. Primer3-new capabilities and interfaces. Nucleic Acids Res. 2012;40(15):1–12. Conesa A, Gà ¶tz S, Garcà ­a-Gà ³mez JM, Terol J, Talà ³n M, Robles M. Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics. 2005;21(18):3674–6. Rajendrakumar P, Biswal AK, Balachandran SM, Sundaram RM. In silico analysis of microsatellites in organellar genomes of major cereals for understanding their phylogenetic relationships. In Silico Biol. 2008;8:87–104. Filiz E, Koc I. In Silico chloroplast SSRs mining of Olea species. BIODIVERSITAS. 2012;13(3):114–7. Kantety R V., La Rota M, Matthews DE, Sorrells ME. Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat. Plant Mol Biol. 2002;48:501–10. Jia XP, Shi YS, Song YC, Wang GY, Wang TY, Li Y. Development of EST-SSR in foxtail millet (Setaria italica). Genet Resour Crop Evol. 2007;54:233–6. Da Maia LC, De Souza VQ, Kopp MM, De Carvalho FIF, De Oliveira AC. Tandem repeat distribution of gene transcripts in three plant families. Genet Mol Biol. 2009;32:822–33. Lewin B, Dover G. Genes v. Oxford University Press Oxford; 1994. Cho YG, Ishii T, Temnykh S, Chen X, Lipovich L, McCouch SR, et al. Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice ( Oryza sativa L.). TAG Theor Appl Genet. 2000;100:713–22. Metzgar D, Bytof J, Wills C. Selection against frameshift mutations limits microsatellite expansion in coding DNA. Genome Res. 2000;10:72–80. Zhao Y, Williams R, Prakash CS, He G. Identification and characterization of gene-based SSR markers in date palm ( Phoenix dactylifera L .). BMC Plant Biology; 2012;12:237 Li D, Deng Z, Qin B, Liu X, Men Z. 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Reflective statement †IRS Essay

After finishing my research project and finally submitting my report, it is necessary that I write a reflective statement on what I have gained from my study. My tutor helped me to change the topic of my research which was â€Å"The link between employee satisfaction and service quality in the hotel industry† to â€Å"How to increase employee retention at the Blue tree hotels†. This helped me be more specific, to focus on a situation on ground, rather than being too general. The broadness of my topic would not help me narrow down to a specific case. This would not help me to draw clear lessons from the project. Being more specific in the project topic statement helped me come up with concise hypothesis for the research. It ultimately made me learn a lot from the project. My methodology was to carry out a filed survey, and sample some of the industries to get information from them. On the advice from my tutor, I changed it to comparative method, which I performed, selecting international hotel chains, such as Marriott, and also using different kinds of case studies in the hospitality industry in order to analyze, compare and opt for the best system and attitude used by them. I got these findings with the intention of implementing them at the Blue Tree Hotels and as a consequence meet the company’s goal. On presenting my idea my tutor, the feedback was negative, but it was helpful as I need pressure to work better. Her guidance led me to the above-discussed process which finally gave me success. At this point I was able to carryout my research with ease and also became considerably successful. The whole process has imparted in me skills and knowledge that are necessary for working on projects, and managing employees for a successful organization or a company. Since the choice of this research project made me to research on employee retention and job satisfaction, it made focus on specific parts of Human Resource management. The ‘employee’ is a very important aspect in an organization, company or institution (Henriques and Sadorsky 199). There are several principles about job satisfaction and retention that I have learned from this project. Furthermore, I have also gained skills in project management, especially in terms of research in order to meet a certain objective. On project management, I was able to sharpen my skills on research. The first thing was the ability to plan my research project (Kumar, 2005). From the topic that my tutor recommended I was able to come up with a research problem. The formulated problem was that the high turn over of employees in various companies has become very expensive. This is fundamentally because the organization or the company loses knowledge and resource they have invested in equipping these people in favor of their competitors (Ramlall, 2003). I was also able to design a model that fit my research project well. I chose on secondary research, which would led me to visit various websites to collect information about how various hotels and hospitality industries had succeeded in maintaining their employees. Secondly, I studied how these companies had achieved their overall goals, based on the principle of employee retention and satisfaction. I chose the highly trusted online data base sites such us Emerald and Ebsco. This formed a good basis of data collection. After identifying this method, I chose the samples to study. These were the best performing companies in this industry. They are Marriot, and Four Seasons. This ultimately led to my writing of the proposal that was accepted by my tutor after following her guidance. In the second step, conducting the study, I was successful in collecting the data from various websites, and studied the data in comparison (Kumar, 2005). I also used books to learn how these companies had applied specific principles in managing this vital resource in the companies. This was in the process of data processing to make them clear during presentation. After successful processing of data, I wrote the report that would easily help in the implementation phase of the project. The report had a very clear explanation on facts about employee retention, loyalty and satisfaction. The aspect I gained through this project is the way to conduct a research and not only stop at the research level but write a report that has conclusions that have clarity and teams can use them to conduct the implementation stage of the project. As I mentioned earlier, I have also come to appreciate the importance of taking care of employees in an organization. Retaining them goes along with how satisfied they are. This becomes a good basis of quality efficient service for the company, institution or organization. In order to maintain competitive advantage, companies have to do all their best to retain the employees they have (Ans and Meganck, 2009). This is because if an organization loses an employee, the quality of the service delivered will be greatly affected, decrease on the efficiency of the service delivery and as a consequence lose guests in addition to profits (Ramlall, 2003). Again, It is clear that in hospitality business, success comes from the loyal employees, since they are committed and trustworthy. This will increase organization’s profitability alongside customer loyalty (Villares and Coelho, 2003). Finally, I learned that increased job satisfaction leads to decreased turnover rates (Smith and Rupp, 2002). From the study I found out that competitive schemes of paying employees, diverse programs of employee motivation, training and reward plans would be very effective in retaining the employees in an organization. Emphatically, such things as opportunities that the companies give in terms of professional growth of the employees also matter. Mutual and hierarchical recognition also are very important in the organization. Lastly the social fabric must be constructed in to encourage teamwork and so cordial relationships among all the workers. This project has made me set for my future career as a manager; for I definitely know that the whole picture of an organization is seen in its employees. Four Seasons rightly confess that, â€Å"Our greatest asset, and the key to our success, is our people† (Four Seasons, 2010). In addition to this I have gained knowledge and skills in research, which will greatly help in project management. This has also sharpened my critic ability, and I will be able to analyze situations keenly, consequently coming up with well judged conclusions.